DIMPL

HELP

Sequence

Please enter a protein sequence in FASTA format or as raw text.

Enzymes

The enzymes cleave according to their endo-peptidase activity. This specificity is displayed as a regular expression when hovering the mouse over each enzyme or chemical reagent name.

Please enter your Job-ID to access previous job:

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Here you are able to create a custom cleavage specificity. The | symbolizes the cleavage point. You are able to define your specificity in the range of six amino acids: four amino acids before and two amino acids after the cleavage site. Please enter the one letter code of your amino acid of choice and add several amino acids in a row without a space. Free boxes are kept unspecific.

Dos:
|

Don'ts:
|

Name:

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DIMPL - your personal disulfide mapping planner. This software can be used optimize a protein digestion strategy for disulfide mapping experiments. DIMPL reports the minimal set of proteases / chemical reagents (selected below) that produces cleavage between each cysteine residue in the target protein sequence (inputted below). Therefore the generation of digestion fragments with possible intra-peptide disulfides is avoided. Fragment sequences and corresponding masses are displayed on the following results page.

Sequence

or enter UniProt accession ID:

Select your enzymes and chemical compounds

 

Try to select a couple of enzymes:

Settings

Miscleavage:

Get results link per mail

Currently disabled.

 

* Your job will be saved for 30 days and will be available via a unique web address presented on the following results page.
Please note that the DIMPL algorithm will process your input without exploring the sequence.

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© 2026 Andreas Kist, Angelika Lampert and Andrias O. O'Reilly. All rights reserved.

How to cite DIMPL? Please use the following publication:
Kist, A. M., Lampert, A., & O'Reilly, A. O. (2018). DIsulfide Mapping PLanner Software Tool. Journal of Computational Biology, 25(4), 430-434.

DIMPL was moved from lampert-lab.com to this here in May 2021.