DIMPL

HELP

Sequence analysis

By selecting a fragment of the sequence the weight is dynamically calculated
By double-clicking on fragments in the fragment table you can combine to fragments and see their weight if they are connected via a disulfide bond.
By hovering on fragments they are highlighted in the sequence above. Red highlighting means that the fragment contains at least one cysteine residue, blue highlighting represents fragments without containing cysteine residue.

Mass calculations

The displayed masses are the average of naturally-occurring isotypes and amino acids are in their protonated form predicted at pH 7.

The mass for any peptide can be calculated by using the mouse to highlight the corresponding region in the full-length protein sequence; the "Show sequence only" link can be used to enable this task.

The mass for two fragments can be calculated by doubling-clicking both entries of interest in the fragment table. The mass is displayed as:

Sum - the combined masses of both fragments without any linkage
Disulfide - the combinded masses of both fragments assuming a single disulfide bond; differs from the "Sum" value by 2 Da, corresponding to the loss of 2 hydrogen atoms
Peptide bond - the combinded masses of both fragments assuming they became covalently linked through a condensation reaction differs from the "Sum" value by 18 Da, corresponding to the loss of a water molecule

Ooops, you got a 404!

You tried to access a site, but this URI is not available.
Perhaps you have a typing mistake or something else.

Return to DIMPL home page

How to cite DIMPL? Please use the following publication:
Kist, A. M., Lampert, A., & O'Reilly, A. O. (2018). DIsulfide Mapping PLanner Software Tool. Journal of Computational Biology, 25(4), 430-434.

DIMPL was moved from lampert-lab.com to this here in May 2021.